The Jean-Nickolaus Tretter Collection in Gay, Lesbian, Bisexual and Transgender Studies houses over 30,000 items in a wide variety of media. The collection is international in scope and has materials in approximately 58 languages. It covers all time periods, from a 4,000-year-old phallic statuette from Egypt to current editions of LGBTQIA periodicals.
Although books are the core of the collection, substantial sections include textiles, glassware, film, music, art works, and three-dimensional objects such as event buttons and furniture. The collection includes unpublished manuscripts, vertical files, and periodicals from all over the world. Among the significant archival holdings are the Jim Chalgren Collection, the personal papers of Professor Toni McNaron, Patrick Scully, Dallas S. Drake, Stuart Ferguson, and Tobias Schneebaum.
The collection also includes archives of the National Log Cabin Republicans, National Education Association GLBT Caucus, the Lesbian Review of Books Archive, Gay/Lesbian Postal Employees Network (PEN) Minneapolis/Saint Paul, Paper Eagles (the employee group of the Minneapolis Star Tribune), the Ramsey County GLBT Employees Network, and the archives of the Twin Cities-GLBT Pride Committee.